Using PCR to Detect Aspergillus and Assess Resistance

Polymerase chain reaction analysis detected Aspergillus species and triazole resistance in respiratory specimens from many patients with negative fungal cultures.

Aspergillus species are associated with several clinical syndromes, including invasive pulmonary aspergillosis (IPA), chronic pulmonary aspergillosis (CPA), and allergic bronchopulmonary aspergillosis (ABPA). Triazole antifungals are recommended for these syndromes, but resistance to these agents is increasing. Currently, detection of antifungal resistance requires a positive culture.

In a study partially funded by the assay manufacturer, researchers examined the use of reverse-transcriptase polymerase chain reaction (PCR) to detect the presence of Aspergillus or Penicillium species in respiratory samples and to assess resistance based on mutations in the CYP51A gene, which encodes the azole target protein lanosterol 14α-demethylase. Bronch…

Reviewing Author

Neil M. Ampel, MD

Disclosures

Editorial BoardsUpToDate

Leadership Positions in Professional SocietiesCoccidioidomycosis Study Group (Past President, Secretary, Treasurer)

Neil M. Ampel, MD

Disclosures

Editorial BoardsUpToDate

Leadership Positions in Professional SocietiesCoccidioidomycosis Study Group (Past President, Secretary, Treasurer)

Using PCR to Detect Aspergillus and Assess Resistance

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Reviewing Author