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Results of antimicrobial susceptibility testing provide fundamental guidance for clinicians prescribing antimicrobial therapy. Increasing evidence suggests that Mueller-Hinton Broth (MHB) — the enriched microbiological testing medium that forms the foundation of clinical in vitro antimicrobial susceptibility testing because it supports the growth of most clinically relevant bacterial pathogens — does not simulate antibiotic activity in vivo. Now, researchers have systematically compared the in vitro activities of numerous antibiotics used clinically against staphylococci, Streptococcus pneumoniae, and various Gram-negative pathogens grown either in various tissue culture or host-mimicking media (e.g., Dulbecco's Modified Eagle Media, modifi…