Genotypic Sequencing to Define Appropriate TB Therapy

Whole-genome sequencing can accurately predict phenotypic susceptibility to first-line agents for M. tuberculosis.

Preventing the emergence of multidrug-resistant Mycobacterium tuberculosis (MTB) is dependent upon having accurate antimicrobial susceptibility information. However, phenotypic susceptibility testing for this pathogen is costly, slow, and requires highly trained laboratory personnel. As whole-genome sequencing (WGS) has become less expensive and more readily available it offers important advantages in identifying drug-resistant MTB isolates. An international team of investigators used 23 collections of isolates from 16 countries — several enriched for antimicrobial resistance — to compare the phenotypic and WGS-determined susceptibility of each to isoniazid, rifampin, ethambutol, and pyrazinamide.

There were 10,209 isolates available with ph…

Reviewing Author

Richard T. Ellison III, MD

Disclosures

Consult/Advisory BoardAcurx Pharmaceuticals, LLC;

Grant/Research SupportDoctors Company Foundation, Massachusetts Department of Public Health

Richard T. Ellison III, MD

Disclosures

Consult/Advisory BoardAcurx Pharmaceuticals, LLC;

Grant/Research SupportDoctors Company Foundation, Massachusetts Department of Public Health

Genotypic Sequencing to Define Appropriate TB Therapy

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Reviewing Author